Large-scale mitochondrial DNA analysis of the domestic goat reveals six haplogroups with high diversity

Get Complete Project Material File(s) Now! » Tools to understand livestock origin and diversity Genetic tools Genetic tools have a central role in many biological investigations. For instance, genetic analysis can provide insights into diverse investigations such as evolutionary histories...

Identification of 5mC and 6mA in the genomic DNA of G. intestinalis

Get Complete Project Material File(s) Now! » Identification of 6mA and 5mC in the genomic DNA of G. intestinalis. In order to study DNA methylation in Giardia we used dot-blots to detect 6mA and 5mC modifications in different strains of...

Recombinant invasive Lactococcus lactis can transfer DNA vaccines either directly to dendritic cells or across an epithelial cell monolayer

Get Complete Project Material File(s) Now! » Fate of plasmid DNA after administration After intramuscular or intradermal injection, the plasmid DNA will transfect somatic cells, such as myocytes and keratinocytes, and/or resident Antigen Presenting Cells (APCs) like dendritic cells (DCs)...

DNA-TFs interplay in the control of gene expression

Get Complete Project Material File(s) Now! » Back to the water looking for ARF ancestors Land plants evolution from algae happened hand in hand with the adaptation to terrestrial stresses and the development of a certain body complexity. Given the...

DNA damage and mutation in stem and progenitor cells in the context of aging and cancer

Get Complete Project Material File(s) Now! » When protection mechanisms fail: acquisition of mutation Despite the numerous mechanisms in place to protect stem cells from harmful effects of DNA damage, studies over the past 10 years revealed the extent to...

The ancient DNA revolution: admixture with archaic hominins

Get Complete Project Material File(s) Now! » Detecting positive selection with allele frequency information There are a multitude of methods to determine if a given genomic region is under positive selection. Here, I discuss the overall concept behind these methods...

DNA REPLICATION MECHANISM AND THE CORRESPONDING KNOWLEDGE

Get Complete Project Material File(s) Now! » Determination of replication timing by log2 ratio of replicated DNA The two sub-methods shown in Fig. 1.5, both use the fluorescence-activated cell sorter (FACS) to select cells based upon the increase in DNA...

DNA extraction and phenotyping the segregating population

Get Complete Project Material File(s) Now! » From anisogamy to morphologically different males and females: evolution of sexual dimorphisms It is largely admitted that the initiation of sexual conflict, sexual selection and “attribution” of sex roles evolved from a cascade...

cDNA target synthesis and fluorescent dye labelling

Get Complete Project Material File(s) Now! » METHODOLOGY Methods for Chapter 3 – Connexin Microarray Chip Design and Validation in Rat and Human Design Oligonucleotide probe design Background An oligonucleotide probe for a cDNA microarray is a single stranded DNA...

Insights into the Biogeographical Patterns of Planktonic Diatom Diversity

Get Complete Project Material File(s) Now! » Habitats and adaptations As prolific phototrophic organisms, diatoms can live in the open ocean, polar waters, tropical waters, all fresh water areas, soil, snow and even glacial ice. During the course of evolution...